ADAMTS20

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA027608

 

Antibody HPA027609

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA027608 HPA027609 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

6 6 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

97 88 

Antigen sequence

DWSPCSASCGHGKTTRQVLCMNYHQPIDENYCDPEVRPLMEQECSLAACP
PAHSHFPSSPVQPSYYLSTNLPLTQKLEDNENQVVHPSVRGNQWRTG
 
STRPCSQRRCWSQDCVQHKGMERGRLNCSTSCERKDSHQRMECTDNQIRQ
VNEIVYNSSTISLTSKNCRNPPCNYIVVTADSSQCANN
 

Matching transcripts

ADAMTS20-001 - ENSP00000374071 [100%]
ADAMTS20-002 - ENSP00000448341 [100%]
ADAMTS20-003 - ENSP00000447427 [100%]
ADAMTS20-201 - ENSP00000378911 [100%]
 ADAMTS20-001 - ENSP00000374071 [100%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
More information
 Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:25 1:30 

Validation IH

Uncertain: Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data Uncertain: Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line U-251MG shows positivity in cytoskeleton (microtubules).
More information
 Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & golgi apparatus.
More information
 

Antibody dilution

1:5 1:7 

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available. Uncertain: The subcellular location is partly supported by literature or no literature is available. 

         Western Blot

Image

  

Description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

214.7, 169.5, 71.7, 70.2 214.7 

Antibody dilution

1:250 1:250 

Validation WB

Non-supportive: Only bands not corresponding to the predicted size Uncertain: Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:500 1:500 

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%). Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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ADAMTS20-001
 
ADAMTS20-002
 
ADAMTS20-003
 
ADAMTS20-201