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SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm, Golgi apparatus, Cytoskeleton (Microtubules)

Additional location(s)

 

Staining summary

Localized to the Golgi apparatus, the cytoplasm and microtubules.

Reliability (APE)

Very low

Antibodies in assay

HPA027608, HPA027609
 

Antibody HPA027608

 

Antibody HPA027609

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoskeleton (Microtubules) Cytoplasm, Golgi apparatus 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoskeleton (Microtubules) Cytoplasm, Golgi apparatus 

Staining characteristics

  

Staining intensity

Strong Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoskeleton (Microtubules) Cytoplasm, Golgi apparatus 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in cytoskeleton (microtubules).
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & golgi apparatus.
 

Summary

Staining of cytoskeleton (microtubules) in all three cell lines with additional staining of nucleus in A-431.   Staining of the Golgi apparatus and cytoplasm in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Microtubules
Golgi apparatus