FKBP2

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA049405

 

Antibody CAB025561

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 R&D systems
 

Product name

HPA049405 MAB4356 

Host species

Rabbit Rat 

Clonality

pAb mAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Protein A/G 

Released in version

12 5 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein 

Length (aa)

29  

Antigen sequence

RKLQIGVKKRVDHCPIKSRKGDVLHMHYT
 
 

Matching transcripts

FKBP2-001 - ENSP00000378046 [100%]
FKBP2-003 - ENSP00000310935 [100%]
FKBP2-004 - ENSP00000398147 [100%]
FKBP2-005 - ENSP00000438749 [100%]
  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

 Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in a subset of lymphoid cells outside reaction centra.
More information
 

Retrieval method

 HIER pH6 

Antibody dilution

 1:2000 

Validation IH

 Supportive: One antibody yielding a staining pattern which is consistent with gene/protein characterization data 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
  

Target mass (kDa)

15.6, 11.3 15.6, 11.3 

Antibody dilution

1:290 1:500 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Non-supportive: Weak band of predicted size but with additional bands of higher intensity also present 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line PC-3 shows positivity in plasma membrane.
More information
  

Antibody dilution

1:57  

Validation IF

Uncertain: The subcellular location is partly supported by literature or no literature is available.  

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
  

Antibody dilution

1:2300  

Validation PA

Uncertain: Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).  
 

ANTIGEN VIEW

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FKBP2-001
 
FKBP2-003
 
FKBP2-004
 
FKBP2-005