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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm

Additional location(s)

 

Staining summary

Localized to the nucleus, cytoplasm and plasma membrane.

Reliability (APE)

Low

Antibodies in assay

HPA026313, HPA026517
 

Antibody HPA026313

 

Antibody HPA026517

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

Spotty  

Staining intensity

Strong Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Weak Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

Spotty  

Staining intensity

Weak Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli, plasma membrane & cytoplasm.
 

Summary

Staining of nucleus and cytoplasm in all three cell lines.   Staining of the nucleus in all three cell lines. Additional staining of cytoplasm and plasma membrane in U-251 MG and A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli
Plasma membrane