The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemical staining of human gall bladder shows distinct membranous and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Summary
Most of the normal tissues displayed weak to moderate cytoplasmic positivity. Strong staining was observed in exocrine pancreas, placenta, Purkinje cells and a subset of lymphoid cells outside reaction centra.
Most normal cells showed weak to moderate cytoplasmic staining combined with membranous positivity in a few cases. Strong immunoreactivity was observed in a subset of lymphoid cells while the liver were negative.
Most normal tissues showed weak to moderate nuclear positivity. Decidual cells, subsets of alveolar cells, gall bladder, large intestine and upper glandular cells of stomach showed strong nuclear immunoreactivity. The liver, cells of CNS, myocytes and most lymphoid cells were negative. Malignant cells were generally negative or weakly stained.
Normal tissues displayed weak to moderate cytoplasmic positivity. Myocytes and Purkinje cells displayed strong cytoplasmic staining. Lymphoid tissue, islets of Langerhans, pneumocytes, trophoblastic cells and prostate were negative.
Validation
Two (or more) antibodies yielding dissimilar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding dissimilar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding dissimilar staining patterns which are consistent with available gene/protein characterization data
The multi-targeting antibody yielding a staining pattern partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data for all of the genes
