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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Plasma membrane, Cytoplasm, Cytoskeleton (Intermediate filaments)

Additional location(s)

 

Staining summary

Localized to cytoplasm, nucleus, plasma membrane and intermediate filaments.

Reliability (APE)

Very low

Antibodies in assay

CAB011464, CAB035990
 

Antibody CAB011464

 

Antibody CAB035990

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Cytoskeleton (Intermediate filaments) Plasma membrane, Cytoplasm 

Staining characteristics

 Granular 

Staining intensity

Weak Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm, Cytoskeleton (Intermediate filaments) Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli, cytoplasm & cytoskeleton (intermediate filaments).
 

Immunofluorescent staining of human cell line U-251MG shows positivity in plasma membrane & cytoplasm.
 

Summary

Staining of nucleus and cytoplasm in all three cell lines. Additional staining of cytoskeleton (intermediate filaments) in U-2 OS and U-251 MG.   Staining of plasma membrane and cytoplasm in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Intermediate filaments
Nucleus but not nucleoli
Plasma membrane