The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Most normal tissues displayed weak to moderate cytoplasmic positivity. Staining pattern was often granular. Strong staining was observed in a fraction of cells in renal tubules and in keratinized layers of squamous epithelia. Liver and urothelial cells were mainly negative.
Immunohistochemical staining of human vulva/anal skin shows strong cytoplasmic positivity in stratum granulosum of the squamous epithelia.
Summary
Most normal tissues displayed weak to moderate cytoplasmic positivity. Staining pattern was often granular. Strong staining was observed in a fraction of cells in renal tubules and in keratinized layers of squamous epithelia. Liver and urothelial cells were mainly negative.
Validation
One antibody yielding a staining pattern which is consistent with gene/protein characterization data
