CCDC14

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA035278

 

Antibody HPA035279

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA035278 HPA035279 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

12 6 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

89 86 

Antigen sequence

TCTDVLRNSFNTSPGVPCSLPKTDISAIPTLQQLGLVNGILPQQGIHKET
DLLKCIQTYLSLFRSHGKETHLDSQTHRSPTQSQPAFLA
 
IIYQALCEHVQTQMSLMNDLTSKNIPNGIPAVPCHAPSHSESQATPHSSY
GLCTSTPVWSLQRPPCPPKVHSEVQTDGNSQFASQG
 

Matching transcripts

CCDC14-001 - ENSP00000395706 [100%]
CCDC14-004 - ENSP00000386866 [100%]
CCDC14-017 - ENSP00000418403 [100%]
CCDC14-022 - ENSP00000418002 [100%]
CCDC14-201 - ENSP00000312031 [100%]
CCDC14-202 - ENSP00000420180 [100%]
 CCDC14-001 - ENSP00000395706 [100%]
CCDC14-004 - ENSP00000386866 [100%]
CCDC14-009 - ENSP00000387962 [100%]
CCDC14-017 - ENSP00000418403 [100%]
CCDC14-022 - ENSP00000418002 [100%]
CCDC14-201 - ENSP00000312031 [100%]
CCDC14-202 - ENSP00000420180 [100%]
CCDC14-010 - ENSP00000414655 [99%]
CCDC14-011 - ENSP00000400957 [99%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
More information
 Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:35 1:1200 

Literature conformity

No avaliable characterization data No avaliable characterization data 

RNA consistency

Not done Not done 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

106.3, 104.0, 101.5, 88.2, 83.6 106.3, 104.0, 101.5, 88.2, 83.6, 35.3, 19.4, 12.6 

Antibody dilution

1:250 1:250 

Validation WB

Uncertain: No bands detected Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:3000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 

         Immunofluorescence

Image

  

Description

Application not done for this antibody. Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm, vesicles & nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

 1:76 

Validation IF

 Uncertain: The subcellular location is partly supported by literature or no literature is available. 
 

ANTIGEN VIEW

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CCDC14-001
 
CCDC14-004
 
CCDC14-009
 
CCDC14-010
 
CCDC14-011
 
CCDC14-017
 
CCDC14-022
 
CCDC14-201
 
CCDC14-202