Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Nucleus but not nucleoli, Vesicles

Additional location(s)

Cytoplasm, Centrosome 

Staining summary

Staining of nucleus, cytoplasm and vesicles in all three cell lines with additional staining of Golgi apparatus in U-2 OS.

Reliability (Single)

Antibodies in assay

HPA035279
 

Antibody HPA035279

 

         Cell line A-431

Toggle channels

 

Location(s)

Nucleus but not nucleoli, Vesicles, Centrosome 

Staining characteristics

 

Staining intensity

Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

Toggle channels

 

Location(s)

Nucleus but not nucleoli, Cytoplasm, Golgi apparatus, Vesicles 

Staining characteristics

 

Staining intensity

Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

Toggle channels

 

Location(s)

Nucleus but not nucleoli, Cytoplasm, Vesicles 

Staining characteristics

 

Staining intensity

Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli, vesicles & centrosome.
 

Summary

Staining of nucleus, cytoplasm and vesicles in all three cell lines with additional staining of Golgi apparatus in U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Centrosome
Cytoplasm
Nucleus but not nucleoli
Endosome