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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Cytoplasm

Additional location(s)

Nucleus but not nucleoli 

Staining summary

Mainly localized to plasma membrane and cytoplasm but also nucleus.

Reliability (APE)

High

Antibodies in assay

HPA023310, HPA027421
 

Antibody HPA023310

 

Antibody HPA027421

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm Nucleus but not nucleoli, Plasma membrane, Cytoplasm, Cell Junctions 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Weak Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Plasma membrane, Cytoplasm Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cytoplasm.
 

Summary

Staining of plasma membrane and cytoplasm in all three cell lines.   Staining of cytoplasm and plasma membrane in all three cell lines. Additional staining of nucleus in U-251 MG and A-431. Additional staining of cell junctions in A-431.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli
Plasma membrane