Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleoli, Nucleus but not nucleoli

Additional location(s)

Centrosome 

Staining summary

Localized to the nucleus and nucleoli. Additionally localized to centrosomes.

Reliability (APE)

Very low

Antibodies in assay

HPA029243, HPA029245
 

Antibody HPA029243

 

Antibody HPA029245

 

         Cell line A-431

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Location(s)

Nucleoli Nucleus but not nucleoli, Cytoplasm, Centrosome 

Staining characteristics

 Granular 

Staining intensity

Strong Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

  Centrosome 

Staining characteristics

   

Staining intensity

  Weak 

Validation

  
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Cell line U-251 MG

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Location(s)

  Centrosome 

Staining characteristics

   

Staining intensity

  Moderate 

Validation

  
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleoli.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in centrosome.
 

Summary

Staining of nucleoli in A-431.   Staining of centrosome in all three cell lines with additional staining of nucleus and cytoplasm in A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Centrosome
Nucleoli
Nucleus but not nucleoli