Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Cytoplasm

Additional location(s)

Nucleoli, Cytoskeleton (Cytokinetic bridge) 

Staining summary

Localized to the nucleus and cytoplasm.

Reliability (APE)

Medium

Antibodies in assay

HPA003221, CAB013520
 

Antibody HPA003221

 

Antibody CAB013520

 

         Cell line A-431

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Location(s)

Nucleus, Nucleoli, Cytoplasm   

Staining characteristics

Smooth   

Staining intensity

Strong   

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
   

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus, Cytoplasm Nucleus but not nucleoli, Cytoplasm, Cytoskeleton (Cytokinetic bridge) 

Staining characteristics

Smooth  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus, Cytoplasm   

Staining characteristics

Smooth   

Staining intensity

Strong   

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
   

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus & cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli, cytoplasm & cytoskeleton (cytokinetic bridge).
 

Summary

Staining of nucleus and cytoplasm in all three cell lines with additional staining of nucleoli in A-431.   Staining of nucleus, cytoplasm and cytoskeleton (cytokinetic bridge) in U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Microtubules
Nucleoli
Nucleus