The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in cortical cells.
Immunohistochemical staining of human placenta shows strong positivity in the brushboarder of syncytiotrophoblasts.
Immunohistochemical staining of human placenta shows membranous positivity in trophoblastic cells.
Summary
A majority of normal tissues showed moderate to strong cytoplasmic and occasional nuclear positivity. Glandular cells displayed membranous staining in several cases. Pancreas and stromal cells were weakly stained.
Strong cytoplasmic positivity was observed in cardiac myocytes and the brushborder of trophoblasts. The intestine, pancreatic ducts, renal tubules, most male genitalia, respiratory and transitional epithelium showed moderate cytoplasmic staining. Remaining normal tissues were generally negative.
Trophoblastic cells and cells of parathyroid gland, endometrium and prostate showed moderate cytoplasmic positivity. The remaining normal tissues were weakly stained or negative.
Validation
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
