GOLGA8Q

ANTIBODY AND ANTIGEN INFORMATION

? »
 

Antibody HPA045397

 

Antibody HPA045527

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA045397 HPA045527 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

10 10 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

26 26 

Antigen sequence

KHGDLREVTLTSSAQGEAREDPLLDK
 
TSDPSKKMWEAGTSLWGGEVPGHRQL
 

Matching transcripts

GOLGA8Q-001 - ENSP00000457904 [100%]
 GOLGA8Q-201 - ENSP00000332482 [81%]
 

Other gene match

GOLGA8I - ENSG00000153666 [92%]
GOLGA8J - ENSG00000179938 [88%]
GOLGA8R - ENSG00000186399 [100%]
GOLGA8M - ENSG00000188626 [92%]
GOLGA8O - ENSG00000206127 [100%]
GOLGA8N - ENSG00000232653 [100%]
GOLGA8K - ENSG00000249931 [96%]
GOLGA8T - ENSG00000261247 [96%]
GOLGA8S - ENSG00000261739 [92%]
GOLGA8H - ENSG00000261794 [92%]
 GOLGA6L4 - ENSG00000184206 [100%]
GOLGA8R - ENSG00000186399 [81%]
GOLGA8M - ENSG00000188626 [85%]
GOLGA8O - ENSG00000206127 [81%]
GOLGA8N - ENSG00000232653 [81%]
 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in paneth cells.
More information
 Immunohistochemical staining of human cervix, uterine shows strong cytoplasmic positivity in squamous epithelial cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:150 1:15 

Validation IH

Uncertain: The multi-targeting antibody yielding a staining pattern partly consistent with limited gene/protein characterization data or there is no available gene/protein characterization data for all or at least one of the genes Uncertain: The multi-targeting antibody yielding a staining pattern partly consistent with limited gene/protein characterization data or there is no available gene/protein characterization data for all or at least one of the genes 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

71.8, 71.6, 71.5, 71.3, 70.4, 67.1, 50.5 42.9, 33.4, 27.3, 13.7 

Antibody dilution

1:250 1:250 

Validation WB

Uncertain: No bands detected Uncertain: No bands detected 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:3000 1:500 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

? »
 
 
 
GOLGA8Q-001
 
GOLGA8Q-201