Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Nucleus but not nucleoli

Additional location(s)

Plasma membrane, Cytoplasm 

Staining summary

Mainly localized to nucleus but also plasma membrane and cytoplasm.

Reliability (APE)

High

Antibodies in assay

HPA006769, HPA006996
 

Antibody HPA006769

 

Antibody HPA006996

 

         Cell line A-431

Toggle channels

 
 

Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Weak Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

Toggle channels

 
 

Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

Toggle channels

 
 

Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli, Plasma membrane, Cytoplasm 

Staining characteristics

Granular Granular 

Staining intensity

Weak Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane & cytoplasm.
 

Summary

Staining of nucleus in all three cell lines with additional staining of cytoplasm in U-251 MG and U-2 OS.   Staining of nuclei and plasma membrane/cytoplasm in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli
Plasma membrane