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SUBCELLULAR LOCATION

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Main location(s)

Cytoskeleton (Actin filaments)

Additional location(s)

Cytoplasm 

Staining summary

Localized to actin filaments and the cytoplasm.

Reliability (APE)

Low

Antibodies in assay

HPA026954, HPA026955
 

Antibody HPA026954

 

Antibody HPA026955

 

         Cell line A-431

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Location(s)

Cytoplasm, Cytoskeleton (Actin filaments)  

Staining characteristics

  

Staining intensity

Moderate Negative 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
No staining.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoplasm, Cytoskeleton (Actin filaments)  

Staining characteristics

  

Staining intensity

Moderate Negative 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
No staining.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm, Cytoskeleton (Actin filaments)  

Staining characteristics

  

Staining intensity

Weak Negative 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
No staining.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm & cytoskeleton (actin filaments).
 

Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Summary

Staining of cytoplasm and cytoskeleton (actin filaments) in all three cell lines.   No staining was observed in any of the three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Actin filaments