Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

Cytoskeleton (Intermediate filaments) 

Staining summary

Staining of nucleus in A-431, cytoskeleton (intermediate filaments) in U-2 OS and staining of both nucleus and intermediate filaments in U-251 MG.

Reliability (Single)

Antibodies in assay

HPA046817
 

Antibody HPA046817

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli 

Staining characteristics

 

Staining intensity

Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Cytoskeleton (Intermediate filaments) 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoskeleton (Intermediate filaments) 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
 

Summary

Staining of nucleus in A-431, cytoskeleton (intermediate filaments) in U-2 OS and staining of both nucleus and intermediate filaments in U-251 MG.  

Validation

The subcellular location is supported by literature.
 

Dictionary

Intermediate filaments
Nucleus but not nucleoli