PUF60

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA045733

 

Antibody HPA052096

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA045733 HPA052096 

Host species

Rabbit Rabbit 

Clonality

pAb pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

9 13 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment 

Length (aa)

93 83 

Antigen sequence

IKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIM
CRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKG
 
AATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAP
GVITGVTPARPPIPVTIPSVGVVNPILASPPTL
 

Matching transcripts

PUF60-001 - ENSP00000434359 [100%]
PUF60-003 - ENSP00000402953 [100%]
PUF60-005 - ENSP00000395417 [100%]
PUF60-011 - ENSP00000433403 [100%]
PUF60-025 - ENSP00000436131 [100%]
PUF60-002 - ENSP00000322036 [82%]
PUF60-004 - ENSP00000322016 [82%]
PUF60-006 - ENSP00000431960 [82%]
PUF60-012 - ENSP00000434863 [82%]
PUF60-013 - ENSP00000437309 [82%]
PUF60-024 - ENSP00000432610 [82%]
 PUF60-001 - ENSP00000434359 [100%]
PUF60-002 - ENSP00000322036 [100%]
PUF60-003 - ENSP00000402953 [100%]
PUF60-004 - ENSP00000322016 [100%]
PUF60-005 - ENSP00000395417 [100%]
PUF60-006 - ENSP00000431960 [100%]
PUF60-023 - ENSP00000432091 [92%]
 

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
More information
 Application not done for this antibody. 

Retrieval method

HIER pH6  

Antibody dilution

1:25  

Literature conformity

Partly consistent with gene/protein characterization data  

RNA consistency

Not done  

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but excluded from the nucleoli.
More information
 Immunofluorescent staining of human cell line MCF7 shows positivity in nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

1:22 1:81 

Validation IF

Supportive: The subcellular location is supported by literature. Supportive: The subcellular location is supported by literature. 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

59.9, 58.2, 57.1, 55.7, 55.4, 54.0, 35.7, 34.5, 28.7, 26.9, 24.8 59.9, 58.2, 57.1, 55.7, 55.4, 54.0, 28.1 

Antibody dilution

1:250 1:400 

Validation WB

Uncertain: No bands detected Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:500 1:3250 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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PUF60-001
 
PUF60-002
 
PUF60-003
 
PUF60-004
 
PUF60-005
 
PUF60-006
 
PUF60-011
 
PUF60-012
 
PUF60-013
 
PUF60-023
 
PUF60-024
 
PUF60-025
 
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