Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Golgi apparatus, Vesicles

Additional location(s)

 

Staining summary

Localized to the nucleus, vesicles and the Golgi apparatus.

Reliability (APE)

Low

Antibodies in assay

HPA001598, HPA018905
 

Antibody HPA001598

 

Antibody HPA018905

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Golgi apparatus Vesicles 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Golgi apparatus Vesicles 

Staining characteristics

  

Staining intensity

Moderate Strong 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Golgi apparatus Vesicles 

Staining characteristics

Spotty  

Staining intensity

Strong Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli & golgi apparatus.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in vesicles.
 

Summary

Staining of Golgi and nucleus but not nucleoli in all three cell lines.   Staining of vesicles in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Golgi apparatus
Nucleus but not nucleoli
Endosome