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SUBCELLULAR LOCATION

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Main location(s)

Nucleoli, Nuclear membrane

Additional location(s)

Plasma membrane, Cytoplasm, Cytoskeleton (Actin filaments) 

Staining summary

Staining of nucleoli, nuclear membrane and cytoplasm in all three cell lines with additional staining of plasma membrane and cytoskeleton (actin filaments) in U-2 OS.

Reliability (Single)

Antibodies in assay

HPA026659
 

Antibody HPA026659

 

         Cell line A-431

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Location(s)

Nucleoli, Nuclear membrane, Cytoplasm 

Staining characteristics

 

Staining intensity

Weak 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleoli, Nuclear membrane, Plasma membrane, Cytoplasm, Cytoskeleton (Actin filaments) 

Staining characteristics

 

Staining intensity

Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleoli, Nuclear membrane, Cytoplasm 

Staining characteristics

 

Staining intensity

Strong 

Validation

Location not consistent with experimental gene/protein characterization data.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli, nuclear membrane, plasma membrane, cytoplasm & cytoskeleton (actin filaments).
 

Summary

Staining of nucleoli, nuclear membrane and cytoplasm in all three cell lines with additional staining of plasma membrane and cytoskeleton (actin filaments) in U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Actin filaments
Nuclear membrane
Nucleoli
Plasma membrane