ACBD4

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA051772

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA051772 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

10 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

78 

Antigen sequence

SAYITEMKLVAQKVIDTVPLGEVAEDMFGYFEPLYQVIPDMPRPPETFLR
RVTGWKEQVVNGDVGAVSEPPCLPKEPA
 

Matching transcripts

ACBD4-001 - ENSP00000465610 [100%]
ACBD4-002 - ENSP00000465484 [100%]
ACBD4-004 - ENSP00000381367 [100%]
ACBD4-005 - ENSP00000466305 [100%]
ACBD4-006 - ENSP00000366154 [100%]
ACBD4-007 - ENSP00000314440 [100%]
ACBD4-201 - ENSP00000405969 [100%]
 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:125 

Validation IH

Uncertain: One antibody yielding a staining pattern and there is no available gene/protein characterization data or the staining pattern is partly consistent with or contradicted by limited gene/protein characterization data 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

37.8, 34.8, 30.3, 29.7 

Antibody dilution

1:290 

Validation WB

Uncertain: Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:2350 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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ACBD4-001
 
ACBD4-002
 
ACBD4-004
 
ACBD4-005
 
ACBD4-006
 
ACBD4-007
 
ACBD4-201