Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Cytoplasm

Additional location(s)

 

Staining summary

Localized to nucleus and cytoplasm.

Reliability (APE)

Very low

Antibodies in assay

CAB013063, HPA014703
 

Antibody CAB013063

 

Antibody HPA014703

 

         Cell line A-431

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Location(s)

 Nucleus, Cytoplasm 

Staining characteristics

 Granular, Spotty 

Staining intensity

Negative Moderate 

Validation

No staining.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

  

Staining characteristics

  

Staining intensity

Negative Negative 

Validation

No staining.
 
No staining.
 

Comment

  

         Cell line U-251 MG

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Location(s)

 Nucleus but not nucleoli, Cytoplasm 

Staining characteristics

 Spotty 

Staining intensity

Negative Moderate 

Validation

No staining.
 
Location not consistent with experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows no positivity.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus & cytoplasm.
 

Summary

No staining was observed in any of the three cell lines.   Staining of nucleus and cytoplasm in U-251 MG and A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is not consistent with literature.
 

Dictionary

Cytoplasm
Nucleus