Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus

Additional location(s)

Cytoplasm 

Staining summary

Staining of nucleoli in all three cell lines with additional staining of nucleus in U-251 MG and U-2 OS and cytoplasm in U-251 MG and A-431.

Reliability (Single)

Antibodies in assay

HPA003156
 

Antibody HPA003156

 

         Cell line A-431

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Location(s)

Nucleoli, Cytoplasm 

Staining characteristics

Granular 

Staining intensity

Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Nucleus, Nucleoli 

Staining characteristics

Granular 

Staining intensity

Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Nucleus, Nucleoli, Cytoplasm 

Staining characteristics

Granular 

Staining intensity

Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus & nucleoli.
 

Summary

Staining of nucleoli in all three cell lines with additional staining of nucleus in U-251 MG and U-2 OS and cytoplasm in U-251 MG and A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus