Fields »

SUBCELLULAR LOCATION

? »

Main location(s)

Nucleus but not nucleoli

Additional location(s)

Nuclear membrane 

Staining summary

Mainly localized to the nucleus. In addition localized to the nuclear membrane.

Reliability (APE)

Low

Antibodies in assay

HPA039498, HPA040101
 

Antibody HPA039498

 

Antibody HPA040101

 

         Cell line A-431

Toggle channels

 
 

Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

Toggle channels

 
 

Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

  

Staining intensity

Weak Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-251 MG

Toggle channels

   

Location(s)

Nucleus but not nucleoli, Nuclear membrane   

Staining characteristics

   

Staining intensity

Strong   

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
   

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli & nuclear membrane.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
 

Summary

Staining of nucleus in all three cell lines, with additional staining of nuclear membrane in U-251 MG and cytoplasm in A-431.   Staining of nucleus in A-431 and U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Nuclear membrane
Nucleus but not nucleoli