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SUBCELLULAR LOCATION

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Main location(s)

Nuclear membrane, Golgi apparatus

Additional location(s)

 

Staining summary

Localized to the Golgi apparatus or nuclear membrane.

Reliability (APE)

Very low

Antibodies in assay

HPA003494, CAB003816
 

Antibody HPA003494

 

Antibody CAB003816

 

         Cell line A-431

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Location(s)

Golgi apparatus Nuclear membrane 

Staining characteristics

  

Staining intensity

Strong Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Golgi apparatus Nuclear membrane 

Staining characteristics

Granular  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Golgi apparatus Nuclear membrane 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data but showing dissimilar staining to ≥1 other antibody.
 
Location not consistent with experimental gene/protein characterization data and showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in golgi apparatus.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nuclear membrane.
 

Summary

Staining of Golgi in all three cell lines.   Staining of nuclear membrane in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is not consistent with literature.
 

Dictionary

Golgi apparatus
Nuclear membrane