Fields »

SUBCELLULAR LOCATION

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Main location(s)

Mitochondria

Additional location(s)

 

Staining summary

Localized to mitochondria.

Reliability (APE)

High

Antibodies in assay

HPA022853, HPA023043, HPA023048
 

Antibody HPA022853

 

Antibody HPA023043

 

Antibody HPA023048

 

         Cell line A-431

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Location(s)

Mitochondria Mitochondria Mitochondria 

Staining characteristics

   

Staining intensity

Strong Strong Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Mitochondria Mitochondria Mitochondria 

Staining characteristics

   

Staining intensity

Strong Strong Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Mitochondria Mitochondria Nucleus but not nucleoli, Mitochondria 

Staining characteristics

   

Staining intensity

Strong Strong Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in mitochondria.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in mitochondria.
 

Immunofluorescent staining of human cell line A-431 shows positivity in mitochondria.
 

Summary

Staining of mitochondria in all three cell lines.   Staining of mitochondria in all three cell lines.   Staining of mitochondria in all three cell lines with additional staining of nucleus in U-251 MG.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Mitochondria