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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Golgi apparatus, Vesicles

Additional location(s)

 

Staining summary

Localized to the plasma membrane, vesicles and the Golgi apparatus.

Reliability (APE)

Low

Antibodies in assay

HPA014704, HPA023890
 

Antibody HPA014704

 

Antibody HPA023890

 

         Cell line A-431

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Location(s)

Plasma membrane, Golgi apparatus Vesicles 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Cell line U-2 OS

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Location(s)

 Vesicles 

Staining characteristics

  

Staining intensity

Negative Moderate 

Validation

No staining.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Plasma membrane Vesicles 

Staining characteristics

  

Staining intensity

Weak Weak 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 
Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in plasma membrane.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in vesicles.
 

Summary

Staining of plasma membrane in U-251 MG and A-431 with additional staining of Golgi in A-431.   Staining of vesicles in all three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Golgi apparatus
Plasma membrane
Endosome