NPIPA7

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA053611

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA053611 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

11 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

26 

Antigen sequence

NRMGHQPPPPTQQHSIIDNSLSLKTP
 

Matching transcripts

NPIPA7-001 - ENSP00000370908 [100%]
NPIPA7-201 - ENSP00000330634 [100%]
 

Other gene match

RP11-231C14.4 - ENSG00000169203 [96%]
NPIPB3 - ENSG00000169246 [96%]
NPIPA1 - ENSG00000183426 [100%]
NPIPA5 - ENSG00000183793 [100%]
NPIPB4 - ENSG00000185864 [96%]
NPIPB15 - ENSG00000196436 [92%]
CTB-134H23.2 - ENSG00000196796 [92%]
NPIPB9 - ENSG00000196993 [92%]
RP11-347C12.1 - ENSG00000198064 [96%]
NPIPB6 - ENSG00000198156 [92%]
NPIPA8 - ENSG00000214940 [100%]
NPIPA7 - ENSG00000214967 [100%]
NPIPA3 - ENSG00000224712 [100%]
NPIPA7 - ENSG00000233024 [100%]
NPIPB7 - ENSG00000233232 [92%]
RP11-166B2.1 - ENSG00000234719 [96%]
NPIPB5 - ENSG00000243716 [96%]
NPIPB11 - ENSG00000254206 [92%]
NPIPA2 - ENSG00000254852 [100%]
NPIPB8 - ENSG00000255524 [92%]
 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:20 

Literature conformity

Consistent with gene/protein characterization data 

RNA consistency

Not done 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

129.2, 126.7, 126.5, 126.0, 108.7, 107.7, 95.9, 95.8, 83.7, 67.1, 65.9, 51.3, 49.8, 49.6, 49.2, 47.7, 47.2, 45.8, 45.6, 42.3, 42.2, 42.1, 40.9, 40.6, 40.1, 40.0, 36.2, 35.3, 16.7 

Antibody dilution

1:220 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:1750 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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NPIPA7-001
 
NPIPA7-201