Fields »

SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Cytoplasm

Additional location(s)

Cytoskeleton (Intermediate filaments) 

Staining summary

Staining of plasma membrane and cytoplasm in all three cell lines with additional staining of cytoskeleton (intermediate filaments) in U-2 OS.

Reliability (Single)

Antibodies in assay

HPA040421
 

Antibody HPA040421

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm 

Staining characteristics

 

Staining intensity

Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Plasma membrane, Cytoplasm, Cytoskeleton (Intermediate filaments) 

Staining characteristics

 

Staining intensity

Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Plasma membrane, Cytoplasm 

Staining characteristics

 

Staining intensity

Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane, cytoplasm & cytoskeleton (intermediate filaments).
 

Summary

Staining of plasma membrane and cytoplasm in all three cell lines with additional staining of cytoskeleton (intermediate filaments) in U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Intermediate filaments
Plasma membrane