Fields »

SUBCELLULAR LOCATION

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Main location(s)

Vesicles

Additional location(s)

Cytoplasm 

Staining summary

Localized to vesicles and the cytoplasm.

Reliability (APE)

High

Antibodies in assay

HPA004872, HPA007728
 

Antibody HPA004872

 

Antibody HPA007728

 

         Cell line A-431

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Location(s)

Cytoplasm, Vesicles Cytoplasm, Vesicles 

Staining characteristics

Granular  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoplasm, Vesicles Vesicles 

Staining characteristics

  

Staining intensity

Strong Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm, Vesicles Cytoplasm, Vesicles 

Staining characteristics

Granular  

Staining intensity

Moderate Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & vesicles.
 

Immunofluorescent staining of human cell line A-431 shows positivity in cytoplasm & vesicles.
 

Summary

Staining of cytoplasm and vesicles in all three cell lines.   Staining of vesicles in all three cell lines with additional staining of cytoplasm in U-251 MG and A-431.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Endosome