RXRA

ANTIBODY AND ANTIGEN INFORMATION

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Antibody CAB004565

 

Antibody CAB005352

 

ANTIBODY INFORMATION

Provider

Santa Cruz Biotechnology
 Santa Cruz Biotechnology
 

Product name

sc-46659 sc-553 

Host species

Mouse Rabbit 

Clonality

mAb msAb 

Purity

Protein A/G Affinity 

Released in version

2 4 

ANTIGEN INFORMATION

Antigen

Recombinant protein Synthetic peptide 

Length (aa)

  

Antigen sequence

 
 

Matching transcripts

  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells and lymphoid cells outside reaction centra.
More information
 Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:75 1:300 

Validation IH

Supportive: Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data Supportive: Two (or more) antibodies yielding similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

50.8, 41.1 50.8, 41.1 

Antibody dilution

1:500 1:500 

Validation WB

Uncertain: No bands detected Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Immunofluorescence

Image

  

Description

 Immunofluorescent staining of human cell line A-431 shows positivity in nucleus & golgi apparatus.
More information
 

Antibody dilution

 1:75 

Validation IF

 Supportive: The subcellular location is supported by literature. 
 

ANTIGEN VIEW

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 Not available