H2AFX

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA041189

 

Antibody HPA051647

 

Antibody CAB012264

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Atlas Antibodies
Sigma-Aldrich
 Abcam plc
 

Product name

HPA041189 HPA051647 ab10475 

Host species

Rabbit Rabbit Rabbit 

Clonality

pAb pAb msAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity purified using the PrEST-antigen as affinity ligand Affinity 

Released in version

9 14 4 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Recombinant protein fragment Synthetic peptide 

Length (aa)

26 26  

Antigen sequence

AVRNDEELNKLLGGVTIAQGGVLPNI
 
PKKTSATVGPKAPSGGKKATQASQEY
 
 

Matching transcripts

H2AFX-001 - ENSP00000434024 [96%]
 H2AFX-001 - ENSP00000434024 [100%]
  

Other gene match

H2AFY2 - ENSG00000099284 [81%]
H2AFY - ENSG00000113648 [85%]
HIST1H2AA - ENSG00000164508 [96%]
HIST1H2AC - ENSG00000180573 [92%]
HIST3H2A - ENSG00000181218 [92%]
HIST2H2AC - ENSG00000184260 [92%]
HIST2H2AB - ENSG00000184270 [100%]
HIST1H2AI - ENSG00000196747 [92%]
HIST1H2AG - ENSG00000196787 [92%]
HIST1H2AD - ENSG00000196866 [92%]
HIST2H2AA4 - ENSG00000203812 [92%]
H2AFJ - ENSG00000246705 [92%]
HIST2H2AA4 - ENSG00000272196 [92%]
HIST1H2AH - ENSG00000274997 [92%]
HIST1H2AK - ENSG00000275221 [92%]
HIST1H2AJ - ENSG00000276368 [92%]
HIST1H2AL - ENSG00000276903 [92%]
HIST1H2AE - ENSG00000277075 [92%]
HIST1H2AB - ENSG00000278463 [92%]
HIST1H2AM - ENSG00000278677 [92%]
   

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.
More information
 Application not done for this antibody. Immunohistochemical staining of human cerebellum shows strong nuclear positivity in cerebellar tissue.
More information
 

Retrieval method

HIER pH6  HIER pH6 

Antibody dilution

1:500  1:500 

Literature conformity

Consistent with extensive gene/protein characterization data  Consistent with extensive gene/protein characterization data 

RNA consistency

Mainly consistent with RNA expression data  Mainly consistent with RNA expression data 

         Immunofluorescence

Image

   

Description

Application not done for this antibody. Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but excluded from the nucleoli.
More information
 Application not done for this antibody. 

Antibody dilution

 1:7  

Validation IF

 Supportive: The subcellular location is supported by experimental gene/protein characterization data, gene silencing, or an independent antibody.  

         Western Blot

Image

   

Description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 213, 118, 83, 50, 33, 26, 18
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

40.1, 39.6, 39.5, 39.2, 15.1, 14.2, 14.1, 14.0, 13.9 15.1 15.1 

Antibody dilution

1:250 1:80 1:500 

Validation WB

Non-supportive: Only bands not corresponding to the predicted size Supportive: Band of predicted size in kDa (+/-20%) with additional bands present Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

   

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. 

Antibody dilution

1:3000 1:550  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

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H2AFX-001