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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoplasm, Cytoskeleton (Microtubules)

Additional location(s)

 

Staining summary

Localized to nucleus, cytoplasm and microtubules

Reliability (APE)

Medium

Antibodies in assay

CAB012264, HPA041189
 

Antibody CAB012264

 

Antibody HPA041189

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Cytoskeleton (Microtubules)  

Staining characteristics

  

Staining intensity

Strong Negative 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
The multi-targeting antibody not yielding a staining pattern.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Cytoskeleton (Microtubules)  

Staining characteristics

  

Staining intensity

Weak Negative 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
The multi-targeting antibody not yielding a staining pattern.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm  

Staining characteristics

  

Staining intensity

Moderate Negative 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
The multi-targeting antibody not yielding a staining pattern.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli, cytoplasm & cytoskeleton (microtubules).
 

Immunofluorescent staining of human cell line A-431 shows no positivity.
 

Summary

Staining of nucleus and cytoplasm in all three cell lines, with additional staining of cytoskeleton (microtubules) in A-431 and U-2 OS.   No staining was observed in any of the three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Microtubules
Nucleus but not nucleoli