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SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli, Cytoplasm, Vesicles

Additional location(s)

 

Staining summary

Localized to the cytoplasm, nucleus and vesicles.

Reliability (APE)

Very low

Antibodies in assay

HPA023369, HPA023940
 

Antibody HPA023369

 

Antibody HPA023940

 

         Cell line A-431

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Location(s)

 Vesicles 

Staining characteristics

  

Staining intensity

Negative Moderate 

Validation

No staining.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoplasm Nucleus but not nucleoli, Cytoplasm, Vesicles 

Staining characteristics

Granular  

Staining intensity

Weak Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm Nucleus but not nucleoli, Cytoplasm, Vesicles 

Staining characteristics

Granular  

Staining intensity

Weak Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 
One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but showing dissimilar staining to ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli, cytoplasm & vesicles.
 

Summary

Granular staining of cytoplasm in U-251 MG and U-2 OS.   Staining of cytoplasm, vesicles and nuclei in U-251 MG and U-2 OS. Staining of vesicles in A-431.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus but not nucleoli
Endosome