Fields »

SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Nucleus but not nucleoli, Cytoskeleton (Microtubules) 

Staining summary

Mainly localized to the cytoplasm. In addition localized to the nucleus and microtubules.

Reliability (APE)

Medium

Antibodies in assay

HPA042086, HPA042104
 

Antibody HPA042086

 

Antibody HPA042104

 

         Cell line A-431

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Location(s)

Cytoplasm Cytoplasm, Cytoskeleton (Microtubules) 

Staining characteristics

  

Staining intensity

Weak Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Cell line U-2 OS

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Location(s)

  Cytoplasm, Cytoskeleton (Microtubules) 

Staining characteristics

   

Staining intensity

  Moderate 

Validation

  
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Cytoplasm Cytoplasm 

Staining characteristics

  

Staining intensity

Weak Weak 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & cytoskeleton (microtubules).
 

Summary

Staining of cytoplasm in A-431 and U-251 MG.   Staining of the cytoplasm in all three cell lines. Additional staining of microtubules in A-431 and U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Microtubules
Nucleus but not nucleoli