The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemical staining of human kidney shows strong cytoplasmic and extracellular positivity in cells of tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic and extracellular positivity in cells in tubules.
Summary
Placental trophoblasts, exocrine pancreatic, cells in proximal tubules and prostate showed moderate cytoplasmic positivity. Subsets of cells in bone marrow were strongly stained. Plasma were distinctly stained. Remaining normal tissues were in general negative.
Cytoplasmic and extracellular positivity was observed in most normal tissues. Strongest staining was displayed in renal tubules, trophoblastic cells, prostate glands, breast and macrophages.
A subset of cells in placenta, bronchus and seminiferus duct displayed strong nuclear and cytoplasmic staining whereas adrenal cortical cells, cells in renal tubuli, skin and male genital tract showed moderate cytoplasmic positivity. Alveolar macrophages displayed distinct cytoplasmic staining. Plasma was positive. The remaining normal tissues were mainly negative.
Validation
Two (or more) antibodies yielding partly similar staining patterns which are consistent with gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are consistent with gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are consistent with gene/protein characterization data
