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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Cytoplasm

Additional location(s)

 

Staining summary

Localized to the cytoplasm and plasma membrane.

Reliability (APE)

High

Antibodies in assay

HPA001399, CAB004307, HPA023884
 

Antibody HPA001399

 

Antibody CAB004307

 

Antibody HPA023884

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm  Plasma membrane, Cytoplasm 

Staining characteristics

Smooth   

Staining intensity

Strong Negative Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
No staining.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Plasma membrane, Cytoplasm   Plasma membrane, Cytoplasm 

Staining characteristics

Smooth    

Staining intensity

Strong   Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
   
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

    

         Cell line U-251 MG

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Location(s)

Cytoplasm   Plasma membrane, Cytoplasm 

Staining characteristics

Granular   Granular 

Staining intensity

Moderate   Moderate 

Validation

One/multiple location(s) with no available experimental gene/protein characterization data or partly supported and partly conflicted, but supported by ≥1 other antibody.
   
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

    

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows no positivity.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane & cytoplasm.
 

Summary

Staining of plasma membrane and/or cytoplasm in all three cell lines.   No staining was observed in any of the three cell lines.   Staining of cytoplasm and plasma membrane in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Plasma membrane