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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Cytoplasm, Cytoskeleton (Actin filaments)

Additional location(s)

 

Staining summary

Localized to cytoplasm, plasma membrane and actin filaments.

Reliability (APE)

High

Antibodies in assay

CAB000356, HPA002925
 

Antibody CAB000356

 

Antibody HPA002925

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm, Cytoskeleton (Actin filaments) Plasma membrane, Cytoplasm, Cytoskeleton (Actin filaments) 

Staining characteristics

  

Staining intensity

Moderate Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Plasma membrane, Cytoplasm, Cytoskeleton (Actin filaments) Plasma membrane, Cytoplasm, Cytoskeleton (Actin filaments) 

Staining characteristics

 Smooth 

Staining intensity

Moderate Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Plasma membrane, Cytoplasm, Cytoskeleton (Actin filaments) Plasma membrane, Cytoplasm, Cytoskeleton (Actin filaments) 

Staining characteristics

 Smooth 

Staining intensity

Moderate Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 
One/multiple location(s) supported by experimental gene/protein characterization data and ≥1 other antibody.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane, cytoplasm & cytoskeleton (actin filaments).
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane, cytoplasm & cytoskeleton (actin filaments).
 

Summary

Staining of cytoplasm, plasma membrane and cytoskeleton (actin filaments) in all three cell lines.   Staining of cytoskeleton(actin filaments), cytoplasm and plasma membrane in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Actin filaments
Plasma membrane