The normal tissue page shows antibody staining and, when available, knowledge-based annotated protein expression in 46 human tissues. The assay and annotation is described here.
This page starts with a summary. The summary data can be based on one or more antibodies (reported by the "Antibodies in assay"-field). If the data is based on one single antibody, the IH validation score for that antibody is reported in the "Reliability (Single)"-field (color-coding described here). If the data is based on more than one antibody, the reliability score for the data is reported as "Reliability (APE)" (color-coding described here).
The tissues and cell types can be ordered by organ, cell type or alphabetically.
For each tissue and cell type, the antibody staining is given with the yellow-scale color-coding (described by the scale in the box to the right). Each available antibody is listed in a separate column and the antibody identifier is available at the bottom of the tissue list. The images and annotations can be accessed by clicking on the tissue name.
When available, a knowledge-based annotated protein expression (APE) is given to the right of the antibody staining values. The level of expression is given by a blue-scale color-coding (described by the scale in the box to the right).
At the very bottom of this page, a summary of the staining from each antibody is given, together with a representative image and the immunohistochemistry validation score for the antibody.
Immunohistochemical staining of human kidney shows moderate extra-cellular positivity in cells in tubules and positivity of plasma in blood vessel.
Immunohistochemical staining of human kidney shows moderate extra-cellular positivity in cells in tubules.
Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in acinar cells.
Immunohistochemical staining of human kidney shows distinct positivity of extracellular material.
Summary
Serum showed moderate positivity. Most normal cells were negative.
Serum showed moderate positivity. Most normal cells were negative.
Most normal tissues were weakly stained or negative.
Alveolar macrophages and subset of cells in pancreas showed strong cytoplasmic staining while hematopoietic cells displayed strong nuclear positivity. Moderate cytoplasmic staining was observed in renal tubuli, intestine and placenta.
Most normal were negative, nevertheless distinct secreted and cytoplasmic staining in a few tissues. Selective staining in subsets of cells in pancreatic islets.
Validation
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
Two (or more) antibodies yielding partly similar staining patterns which are partly consistent with gene/protein characterization data or consistent with limited gene/protein characterization data
Two (or more) antibodies yielding similar staining patterns which are consistent with available gene/protein characterization data
