Fields »

SUBCELLULAR LOCATION

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Main location(s)

Focal Adhesions

Additional location(s)

Nucleus, Nucleoli 

Staining summary

Mainly localized to focal adhesion sites but also to the nucleus and nucleoli.

Reliability (APE)

Medium

Antibodies in assay

HPA001239, HPA001349
 

Antibody HPA001239

 

Antibody HPA001349

 

         Cell line A-431

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Location(s)

  Nucleus, Nucleoli, Focal Adhesions 

Staining characteristics

   

Staining intensity

  Moderate 

Validation

  
The multi-targeting antibody yielding a staining pattern partly consistent with available gene/protein characterization data for all of the genes.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Focal Adhesions Focal Adhesions 

Staining characteristics

Granular Granular 

Staining intensity

Moderate Weak 

Validation

The multi-targeting antibody yielding a staining pattern partly consistent with available gene/protein characterization data for all of the genes.
 
The multi-targeting antibody yielding a staining pattern partly consistent with available gene/protein characterization data for all of the genes.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Focal Adhesions Nucleus, Nucleoli, Focal Adhesions 

Staining characteristics

Fibrous, Spotty  

Staining intensity

Moderate Moderate 

Validation

The multi-targeting antibody yielding a staining pattern partly consistent with available gene/protein characterization data for all of the genes.
 
The multi-targeting antibody yielding a staining pattern partly consistent with available gene/protein characterization data for all of the genes.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus but not nucleoli, cytoplasm & focal adhesions.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in nucleus, nucleoli & focal adhesions.
 

Summary

Staining of focal adhesion sites, cytoplasm and nucleus in U-251 MG and A-431.   Staining of focal adhesion sites in all three cell lines. Additional staining of nucleoli and nucleus in U-251MG and A-431.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Focal adhesions
Nucleoli
Nucleus