CFD

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA052799

 

Antibody CAB016383

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Santa Cruz Biotechnology
 

Product name

HPA052799 sc-47683 

Host species

Rabbit Mouse 

Clonality

pAb mAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Protein A/G 

Released in version

14 4 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Native protein 

Length (aa)

54  

Antigen sequence

GIVNHAGRRPDSLQHVLLPVLDRATCNRRTHHDGAITERLMCAESNRRDS
CKGD
 
 

Matching transcripts

CFD-001 - ENSP00000332139 [100%]
CFD-002 - ENSP00000468253 [100%]
  

Other gene match

  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Application not done for this antibody. Immunohistochemical staining of human kidney shows cytoplasmic and extra cellular positivity in cells in tubules.
More information
 

Retrieval method

 HIER pH6 

Antibody dilution

 1:35 

Literature conformity

 Partly consistent with extensive gene/protein characterization data 

RNA consistency

 Mainly not consistent with RNA expression data 

         Immunofluorescence

Image

  

Description

Immunofluorescent staining of human cell line PC-3 shows positivity in plasma membrane.
More information
 Application not done for this antibody. 

Antibody dilution

1:55  

Validation IF

Uncertain: The subcellular location is partly supported by experimental gene/protein characterization data, or no such data is available.  

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

27.9, 27.0 27.9, 27.0 

Antibody dilution

1:270 1:500 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) Supportive: Single band corresponding to the predicted size in kDa (+/-20%) 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. 

Antibody dilution

1:2200  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

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CFD-001
 
CFD-002