HIST4H4

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA042201

 

Antibody CAB011503

 

Antibody CAB037279

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 Abcam plc
 SIGMA
 

Product name

HPA042201 ab17339 H9164 

Host species

Rabbit Rabbit Rabbit 

Clonality

pAb msAb msAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity Affinity 

Released in version

10 4 7 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Synthetic peptide Synthetic peptide 

Length (aa)

25   

Antigen sequence

GKGLGKGGAKRHRKVLRDNIQGITK
 
 
 

Matching transcripts

HIST4H4-002 - ENSP00000443017 [100%]
   

Other gene match

HIST1H4H - ENSG00000158406 [100%]
HIST1H4C - ENSG00000197061 [100%]
HIST1H4J - ENSG00000197238 [100%]
HIST2H4B - ENSG00000270276 [100%]
HIST2H4A - ENSG00000270882 [100%]
HIST1H4K - ENSG00000273542 [100%]
HIST1H4F - ENSG00000274618 [100%]
HIST1H4L - ENSG00000275126 [100%]
HIST1H4G - ENSG00000275663 [92%]
HIST1H4I - ENSG00000276180 [100%]
HIST1H4E - ENSG00000276966 [100%]
HIST1H4D - ENSG00000277157 [100%]
HIST1H4A - ENSG00000278637 [100%]
HIST1H4B - ENSG00000278705 [100%]
   

ANTIBODY VALIDATION

         Immunohistochemistry

Image

   

Description

Immunohistochemical staining of human oral mucosa shows strong nuclear positivity in fraction of squamous epithelial cells.
More information
 Immunohistochemical staining of human liver shows strong nuclear positivity in hepatocytes.
More information
 Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 HIER pH6 

Antibody dilution

1:150 1:100 1:700 

Literature conformity

Consistent with extensive gene/protein characterization data Consistent with extensive gene/protein characterization data Consistent with extensive gene/protein characterization data 

RNA consistency

No internal RNA expression data available for correlation No internal RNA expression data available for correlation No internal RNA expression data available for correlation 

         Immunofluorescence

Image

   

Description

Application not done for this antibody. Application not done for this antibody. Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

  1:175 

Validation IF

  Supportive: The subcellular location is supported by experimental gene/protein characterization data, gene silencing, or an independent antibody. 

         Western Blot

Image

   

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 213, 118, 83, 50, 33, 26, 18
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

11.4, 11.0 11.4 11.4 

Antibody dilution

1:250 1:500 1:500 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) Uncertain: No bands detected Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

   

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. Application not done for this antibody. 

Antibody dilution

1:3000   

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen.   
 

ANTIGEN VIEW

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HIST4H4-002