HIST4H4

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA042201

 

Antibody CAB037279

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 SIGMA
 

Product name

HPA042201 H9164 

Host species

Rabbit Rabbit 

Clonality

pAb msAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand Affinity 

Released in version

10 7 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment Synthetic peptide 

Length (aa)

25  

Antigen sequence

GKGLGKGGAKRHRKVLRDNIQGITK
 
 

Matching transcripts

HIST4H4-001 - ENSP00000350767 [100%]
HIST4H4-002 - ENSP00000443017 [100%]
  

Other gene match

HIST1H4B - ENSG00000124529 [100%]
HIST1H4G - ENSG00000124578 [92%]
HIST1H4H - ENSG00000158406 [100%]
HIST2H4B - ENSG00000182217 [100%]
HIST2H4A - ENSG00000183941 [100%]
HIST1H4D - ENSG00000188987 [100%]
HIST1H4A - ENSG00000196176 [100%]
HIST1H4C - ENSG00000197061 [100%]
HIST1H4J - ENSG00000197238 [100%]
HIST1H4K - ENSG00000197914 [100%]
HIST1H4F - ENSG00000198327 [100%]
HIST1H4I - ENSG00000198339 [100%]
HIST1H4E - ENSG00000198518 [100%]
HIST1H4L - ENSG00000198558 [100%]
  

ANTIBODY VALIDATION

         Immunohistochemistry

Image

  

Description

Immunohistochemical staining of human oral mucosa shows strong nuclear positivity in fraction of squamous epithelial cells.
More information
 Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 HIER pH6 

Antibody dilution

1:150 1:700 

Literature conformity

Consistent with extensive gene/protein characterization data Consistent with extensive gene/protein characterization data 

RNA consistency

Not done Not done 

         Western Blot

Image

  

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

11.4, 11.0 11.4 

Antibody dilution

1:250 1:500 

Validation WB

Supportive: Single band corresponding to the predicted size in kDa (+/-20%) Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 

         Protein array

Image

  

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 Application not done for this antibody. 

Antibody dilution

1:3000  

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen.  
 

ANTIGEN VIEW

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HIST4H4-001
 
HIST4H4-002