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SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Cytoskeleton (Actin filaments)

Additional location(s)

 

Staining summary

Mainly localized to the nucleus. In addition localized to actin filaments.

Reliability (APE)

High

Antibodies in assay

CAB037279, HPA042201
 

Antibody CAB037279

 

Antibody HPA042201

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Nucleus, Cytoskeleton (Actin filaments) 

Staining characteristics

Smooth  

Staining intensity

Strong Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
The multi-targeting antibody yielding a staining pattern partly consistent with available gene/protein characterization data for all of the genes.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli Nucleus, Nucleoli, Cytoskeleton (Actin filaments) 

Staining characteristics

Smooth  

Staining intensity

Strong Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
The multi-targeting antibody yielding a staining pattern partly consistent with available gene/protein characterization data for all of the genes.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli Nucleus, Cytoskeleton (Actin filaments) 

Staining characteristics

Smooth Spotty 

Staining intensity

Strong Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
The multi-targeting antibody yielding a staining pattern partly consistent with available gene/protein characterization data for all of the genes.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus, nucleoli & cytoskeleton (actin filaments).
 

Summary

Staining of nucleus in all three cell lines.   Staining of nucleus and cytoskeleton (actin filaments) in all three cell lines with additional staining of nucleoli in U-2 OS.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Actin filaments
Nucleus