FAM25G

ANTIBODY AND ANTIGEN INFORMATION

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Antibody HPA044808

 

ANTIBODY INFORMATION

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA044808 

Host species

Rabbit 

Clonality

pAb 

Purity

Affinity purified using the PrEST-antigen as affinity ligand 

Released in version

10 

ANTIGEN INFORMATION

Antigen

Recombinant protein fragment 

Length (aa)

51 

Antigen sequence

EVVGHAKETGEKAIAEAIKKAQESGDKKMKEITETVTNTVTNAITHAAES
L
 

Matching transcripts

FAM25G-001 - ENSP00000407749 [100%]
 

Other gene match

FAM25A - ENSG00000188100 [100%]
FAM25C - ENSG00000188279 [100%]
FAM25B - ENSG00000189090 [100%]
FAM25E - ENSG00000231122 [98%]
 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human breast shows moderate cytoplasmic positivity in glandular cells and myoepithelial cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:600 

Validation IH

Uncertain: The multi-targeting antibody yielding a staining pattern partly consistent with limited gene/protein characterization data or there is no available gene/protein characterization data for all or at least one of the genes 

         Western Blot

Image

 

Description

 

Target mass (kDa)

18.3, 9.3 

Antibody dilution

1:250 

Validation WB

Non-supportive: Only bands not corresponding to the predicted size 

         Protein array

Image

 

Description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
More information
 

Antibody dilution

1:12000 

Validation PA

Supportive: Pass with single peak corresponding to interaction only with its own antigen. 
 

ANTIGEN VIEW

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FAM25G-001