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SUBCELLULAR LOCATION

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Main location(s)

Cytoskeleton (Microtubules)

Additional location(s)

Nucleoli, Plasma membrane, Cytoplasm 

Staining summary

Mainly localized to microtubules. In addition localized to cytoplasm and plasma membrane.

Reliability (APE)

Medium

Antibodies in assay

CAB016685, CAB022077
 

Antibody CAB016685

 

Antibody CAB022077

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm, Cytoskeleton (Microtubules) Nucleoli, Cytoskeleton (Microtubules) 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) in cytoplasm (e.g. golgi, mitochondria) supported by experimental evidence for cytoplasmic localization.
 

Comment

  

         Cell line U-2 OS

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Location(s)

Cytoplasm, Cytoskeleton (Microtubules) Nucleoli, Cytoskeleton (Microtubules) 

Staining characteristics

  

Staining intensity

Strong Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) in cytoplasm (e.g. golgi, mitochondria) supported by experimental evidence for cytoplasmic localization.
 

Comment

  

         Cell line U-251 MG

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Location(s)

Cytoplasm, Cytoskeleton (Microtubules) Nucleoli, Cytoskeleton (Microtubules) 

Staining characteristics

  

Staining intensity

Moderate Weak 

Validation

Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
One/multiple location(s) in cytoplasm (e.g. golgi, mitochondria) supported by experimental evidence for cytoplasmic localization.
 

Comment

  

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in plasma membrane, cytoplasm & cytoskeleton (microtubules).
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli & cytoskeleton (microtubules).
 

Summary

Staining of cytoskeleton (microtubules) and cytoplasm in all three cell lines, with additional staining of plasma membrane in A-431.   Staining of cytoskeleton (microtubules) and nucleoli in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Microtubules
Nucleoli
Plasma membrane