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SUBCELLULAR LOCATION

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Main location(s)

Plasma membrane, Cytoplasm

Additional location(s)

 

Staining summary

Localized to the plasma membrane and cytoplasm.

Reliability (APE)

High

Antibodies in assay

HPA003267, HPA003326, CAB004352
 

Antibody HPA003267

 

Antibody HPA003326

 

Antibody CAB004352

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm Cytoplasm 

Staining characteristics

 Smooth  

Staining intensity

Strong Strong Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Plasma membrane, Cytoplasm Nucleoli, Plasma membrane, Cytoplasm  

Staining characteristics

   

Staining intensity

Moderate Strong Negative 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
Multiple locations partly supported (at least one) by experimental gene/protein characterization data.
 
No staining.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Plasma membrane, Cytoplasm Plasma membrane, Cytoplasm Cytoplasm 

Staining characteristics

Granular Smooth  

Staining intensity

Moderate Moderate Weak 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line U-251MG shows positivity in plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in plasma membrane & cytoplasm.
 

Immunofluorescent staining of human cell line U-251MG shows positivity in cytoplasm.
 

Summary

Staining of plasma membrane and cytoplasm in all three cell lines.   Staining of plasma membrane and cytoplasm in all three cell lines with additional staining of nucleoli in U-2 OS.   Staining of cytoplasm in U-251 MG and A-431.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Cytoplasm
Plasma membrane