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SUBCELLULAR LOCATION

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Main location(s)

Cytoplasm

Additional location(s)

Plasma membrane, Cytoskeleton (Actin filaments) 

Staining summary

Staining of cytoplasm in all three cell lines. Additional staining of plasma membrane in A-431 and cytoskeleton (actin filaments) and plasma membrane in U-2 OS.

Reliability (Single)

Antibodies in assay

HPA042515
 

Antibody HPA042515

 

         Cell line A-431

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Location(s)

Plasma membrane, Cytoplasm 

Staining characteristics

 

Staining intensity

Strong 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-2 OS

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Location(s)

Plasma membrane, Cytoplasm, Cytoskeleton (Actin filaments) 

Staining characteristics

 

Staining intensity

Moderate 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Cell line U-251 MG

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Location(s)

Cytoplasm 

Staining characteristics

 

Staining intensity

Weak 

Validation

Not decisive - One/multiple location(s) where experimental gene/protein characterization data is partly supporting and partly conflicting.
 

Comment

 

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in plasma membrane, cytoplasm & cytoskeleton (actin filaments).
 

Summary

Staining of cytoplasm in all three cell lines. Additional staining of plasma membrane in A-431 and cytoskeleton (actin filaments) and plasma membrane in U-2 OS.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Actin filaments
Plasma membrane