Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus but not nucleoli

Additional location(s)

 

Staining summary

The protein is localized to the nucleus but excluded from the nucleoli.

Reliability (APE)

High

Antibodies in assay

HPA023447, HPA023571, HPA025941
 

Antibody HPA023447

 

Antibody HPA023571

 

Antibody HPA025941

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

Speckle Spotty  

Staining intensity

Strong Moderate Strong 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli Nucleus but not nucleoli 

Staining characteristics

   

Staining intensity

Strong Weak Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli 

Staining characteristics

 Aggregate  

Staining intensity

Strong Weak Moderate 

Validation

One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 
One/multiple location(s) supported by experimental gene/protein characterization data.
 

Comment

   

         Antibody summary


Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
 

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.
 

Summary

Staining of nucleus in all three cell lines with additional staining of cytoplasm in U-2 OS.   Staining of nuclei but not nucleoli in all three cell lines with additional staining pf cytoplasm in U-251 MG.   Staining of nucleus in all three cell lines.  

Validation

The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 
The subcellular location is supported by literature.
 

Dictionary

Nucleus but not nucleoli