DDX39B

ANTIBODY AND ANTIGEN INFORMATION

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Antibody CAB034012

 

ANTIBODY INFORMATION

Provider

Santa Cruz Biotechnology
 

Product name

sc-68342 

Host species

Rabbit 

Clonality

pAb 

Purity

Protein A/G 

Released in version

6 

ANTIGEN INFORMATION

Antigen

Recombinant protein 

Length (aa)

 

Antigen sequence

 

Matching transcripts

 

Other gene match

 

ANTIBODY VALIDATION

         Immunohistochemistry

Image

 

Description

Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
More information
 

Retrieval method

HIER pH6 

Antibody dilution

1:125 

Validation IH

Supportive: One antibody yielding a staining pattern which is consistent with gene/protein characterization data 

         Immunofluorescence

Image

 

Description

Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm & nucleus but excluded from the nucleoli.
More information
 

Antibody dilution

1:31 

Validation IF

Supportive: The subcellular location is supported by literature. 

         Western Blot

Image

 

Description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
More information
 

Target mass (kDa)

50.7, 49.0, 48.8, 40.6, 32.9, 28.3, 27.7, 26.7, 26.5, 25.9, 21.9, 21.7, 20.5, 16.5, 14.9, 14.7, 14.3, 13.5 

Antibody dilution

1:500 

Validation WB

Supportive: Band of predicted size in kDa (+/-20%) with additional bands present 
 

ANTIGEN VIEW

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 Not available