Fields »

SUBCELLULAR LOCATION

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Main location(s)

Nucleus, Nucleus but not nucleoli

Additional location(s)

Cytoplasm, Vesicles 

Staining summary

Mainly localized to the nucleus. In addition localized to the cytoplasm and vesicles.

Reliability (APE)

Low

Antibodies in assay

HPA027377, HPA028084, HPA029801
 

Antibody HPA027377

 

Antibody HPA028084

 

Antibody HPA029801

 

         Cell line A-431

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Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli   

Staining characteristics

Speckle    

Staining intensity

Moderate Strong   

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
   

Comment

    

         Cell line U-2 OS

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Location(s)

Nucleus but not nucleoli, Cytoplasm Nucleus but not nucleoli   

Staining characteristics

Speckle    

Staining intensity

Moderate Weak   

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
   

Comment

    

         Cell line U-251 MG

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Location(s)

Nucleus but not nucleoli, Cytoplasm, Vesicles Nucleus but not nucleoli   

Staining characteristics

Speckle    

Staining intensity

Strong Weak   

Validation

One/multiple location(s) with no available experimental gene/protein characterization data.
 
One/multiple location(s) with no available experimental gene/protein characterization data.
   

Comment

    

         Antibody summary


Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli & cytoplasm.
 

Immunofluorescent staining of human cell line A-431 shows positivity in nucleus but not nucleoli.
   

Summary

Staining of cytoplasm and speckled staining of nucleus in all three cell lines, with additional staining of vesicles in U-251 MG.   Staining of nucleus in all three cell lines   No staining was observed in the three cell lines.  

Validation

The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 
The subcellular location is partly supported by literature or no literature is available.
 

Dictionary

Cytoplasm
Nucleus
Nucleus but not nucleoli
Endosome